Protein Method Blocking Buffers
Teknova 20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 8 pack.
20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 8 pack.
IBA LifeSciences Biotin Blocking Buffer; 2 ml
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins such as the biotin carboxyl carrier protein (BCCP, 22 kDa) of E. coli. Biotin Blocking Buffer is recommended for blocking such biotinylated proteins on western blots prior to detection of Strep-tagII or Twin-Strep-tag fusion proteins with Strep-Tactin or Strep-TactinXT conjugates to prevent the detection of false positive bands. The buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins. Please note that usage of BioLock is not necessary when using StrepMAB-Classic, since this antibody does not bind to biotinylated proteins.
